I had to make LB plates but something didn’t look right. After autoclaving it, I knew things were supposed to turn into a solid when it cooled in the petri dish, but as of now it just looked too liquidy (hows that for a science term). Swirl swirl and swirl some more, but liquid it remained. Ten good minutes had passed and it showed no signs of solidifying. Finally the lab manager asked me,
“What are you doing?”
“Trying to get these plates to solidify so I can go home.”
“Did you add agar?”
Whomp Whomp. Who knew you had to add agar to the LB powder. The equivalent is me trying to make jello, swirling around the powder in the water, but without heating it. Just sitting there all ho hum.
Ladies and gentlemen, I was GREEN. Luckily, one, making plates wasn’t the main part of my job, two, I was a quick learner, and three, when I innocuously screwed up, I did it in a way that made the lab manager cry (from laughter). She was a wonderful lab mom in that respect (now she is retired and I hope enjoying every minute of it).
This was my first lab environment. This is where I developed my lab chops. This was my first scientific family.
The boss (AKA the Chair of Microbiology and Immunology) was a “tell it like it is” kind of guy. I always knew where I stood with him because he wasn’t afraid to tell you, whether you asked or not. I cannot tell you how valuable this was for me as a young scientist. I was in the “kid” stage and although I knew I wanted to be a scientist, I had no idea how to get there (or where “there” was). Hence I needed his direction.
I needed his statements like:
“Give journal club as a technician.”
“You are not ready to apply to graduate school this year, but you will be next year.” #truth
“Take this graduate class, it will grow hair on your chest.”
Yes, he encouraged me to take classes while I was in his lab. He reasoned that if I could already pass the classes, the admissions committee would have few things to say against me, and that one I joined graduate school, I would already have a few credits so I could get more done in my rotations. He prepared me to do my job and prepared me to leave at the same time. Yes, I was fortunate to be in his lab, but also this is a tale to you the reader to make sure to try an identify the type of mentor you are getting involved with. I learned more than I thought from him, I realize that now and I remain grateful.
One of the classes he suggested that I take was advance molecular biology. This class had my first “I have to study this topic moment” of my scientific life and it fundamentally changed the course of my science career. I’ll share that in my next post. For now, I’m at the Biometals meeting at Duke University.
Wow you made it this far, good for you!!! Want to do something fun? Tweet me @blacksciblog and tell me what you think the topic was or to tell me what that topic was for you.